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BRAF

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.
  • Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).
  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using BRAF mouse mAb with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using BRAF mouse mAb with DAB staining.

图片

  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.

  • Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using BRAF mouse mAb with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using BRAF mouse mAb with DAB staining.

Mouse Antibody to BRAF
Catalog#   :  32150
Size/Concentration: 50ul100ul
Price: 12802180
Instruction:
Specification
Entrez GeneID673
AliasesNS7; B-raf; BRAF1; RAFB1; B-RAF1
Clone#2A10D7
MW84.4kda
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman, Rat
ImmunogenPurified recombinant fragment of human BRAF (AA: 299-447) expressed in HEK293-6e.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

References
1,Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31105-31113.
2,Sci Rep. 2020 Oct 9;10(1):16943.
Sequence

299-447


Diagram
Elisa

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3) T47D (4) HePG2 (5) HL-60 (6) and PC-12 (7) cell lysate.

Flow cytometric

Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

 

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using BRAF mouse mAb with DAB staining.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using BRAF mouse mAb with DAB staining.

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