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BRAF

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3), T47D (4), HePG2 (5), NIH/3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.
  • Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).
  • Flow cytometric analysis of Hela cells using BRAF mouse mAb (green) and negative control (red).
  • Flow cytometric analysis of HepG2 cells using BRAF mouse mAb (green) and negative control (red).
  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using BRAF mouse mAb with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using BRAF mouse mAb with DAB staining.

图片

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    Elisa

    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

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    Western Blot

    Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3), T47D (4), HePG2 (5), NIH/3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.

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    Flow cytometric

    Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

  • fff
    Flow cytometric

    Flow cytometric analysis of Hela cells using BRAF mouse mAb (green) and negative control (red).

  • fff
    Flow cytometric

    Flow cytometric analysis of HepG2 cells using BRAF mouse mAb (green) and negative control (red).

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using BRAF mouse mAb with DAB staining.

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using BRAF mouse mAb with DAB staining.

Mouse Antibody to BRAF
Catalog#   :  32151
Size/Concentration： 50ul100ul
Price:￥ 12802180
Instruction:
Specification
Entrez GeneID673
AliasesNS7; B-raf; BRAF1; RAFB1; B-RAF1
Clone#3B8B12
MW84.4kda
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Monkey
ImmunogenPurified recombinant fragment of human BRAF (AA: 299-447) expressed in HEK293-6e.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

References
1，Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31105-31113.
2,Sci Rep. 2020 Oct 9;10(1):16943.
Sequence

299-447


Diagram
Elisa

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Western blot analysis using BRAF mouse mAb against Hela (1), HT-29 (2), MOLT4 (3), T47D (4), HePG2 (5), NIH/3T3 (6), PC-12 (7), and COS-7 (8) cell lysate.

Flow cytometric

Flow cytometric analysis of BEL-7402 cells using BRAF mouse mAb (green) and negative control (red).

 

Flow cytometric

Flow cytometric analysis of Hela cells using BRAF mouse mAb (green) and negative control (red).

Flow cytometric

Flow cytometric analysis of HepG2 cells using BRAF mouse mAb (green) and negative control (red).

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using BRAF mouse mAb with DAB staining.

 

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using BRAF mouse mAb with DAB staining.

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