Specification
MW | 26 kDa |
Host/Isotype | Immunogen-specific rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Fusion proteins containing the GST Tag |
Immunogen | Full length fusion protein |
Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Application
ELISA | 1/5000-1/10000 |
WB | 1/2000-1/10000 |
Description
GST (Glutathione S-Transferase) is a 26kDa protein which was widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Diagram
Western Blot |
Gel: 8%SDS-PAGE, Lysate: 0.005/0.01/0.05/0.1 μg, Lane 1-4: 0.005/0.01/0.05/0.1 μg Recombinant GST tag protein, Primary antibody: P00016(GST Tag Antibody) at dilution 1/4000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds |