Specification
Swissprot | P42212 |
MW | 27 kDa |
Host/Isotype | Immunogen-specific rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | GFP transfected cells or Pure GFP protein |
Immunogen | Fusion protein of Aequorea victoria GFP |
Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Application
ELISA | 1/5000-1/10000 |
WB | 1/5000-1/20000 |
Description
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.
Diagram
Western Blot |
Gel: 12%SDS-PAGE, Lysate: 0.001/0.025/0.005/0.008/0.01/0.015/0.02 μg, Lane 1-7: 0.001/0.025/0.005/0.008/0.01/0.015/0.02 μg Green fluorescent protein, Primary antibody: P00008(GFP Antibody) at dilution 1/4000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds |