Specification
Aliases | HA epitope tag, Hemagglutinin Tag |
MW | 50 kDa |
Host/Isotype | Immunogen-specific rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Fusion proteins containing the HA Tag |
Immunogen | Synthetic peptide: YPYDVPDYA conjugated to KLH. |
Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Application
ELISA | 1/5000-1/10000 |
WB | 1/5000-1/20000 |
Description
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Diagram
Western Blot |
Gel: 8%SDS-PAGE, Lysate: 1/0.5 μg, Lane 1-2: Fusion proteins containing the HA Tag, Primary antibody: P00004(HA Tag Antibody) at dilution 1/4000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 5 seconds |