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GOLGA2

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Western blot analysis using GOLGA2 mouse mAb against Jurkat (1) cell lysate.
  • Immunofluorescence analysis of Hela cells using GOLGA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
  • Flow cytometric analysis of Hela cells using GOLGA2 mouse mAb (green) and negative control (red).
  • Immunohistochemical analysis of paraffin-embedded human colon tissues using GOLGA2 mouse mAb with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded human brain tissues using GOLGA2 mouse mAb with DAB staining.

图片

  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Western blot analysis using GOLGA2 mouse mAb against Jurkat (1) cell lysate.

  • Immunofluorescence analysis of Hela cells using GOLGA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Flow cytometric analysis of Hela cells using GOLGA2 mouse mAb (green) and negative control (red).

  • Immunohistochemical analysis of paraffin-embedded human colon tissues using GOLGA2 mouse mAb with DAB staining.

  • Immunohistochemical analysis of paraffin-embedded human brain tissues using GOLGA2 mouse mAb with DAB staining.

Mouse Antibody to GOLGA2
Catalog#   :  32171
Size/Concentration: 50ul100ul
Price: 12802180
Instruction:
Specification
Entrez GeneID2801
AliasesGM130
Clone#2D5D11
MW113kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human GOLGA2 (AA: 1-205) expressed in HEK293-6e cells supernatant.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/50 - 1/200
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

References
1.Sci Rep. 2015 Aug 28;5:13324.
2.Cell Cycle. 2015;14(8):1139-47.
Sequence

1-205


Diagram
Elisa

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Western blot analysis using GOLGA2 mouse mAb against Jurkat (1) cell lysate.

Immunofluorescence

Immunofluorescence analysis of Hela cells using GOLGA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

 

Flow cytometric

Flow cytometric analysis of Hela cells using GOLGA2 mouse mAb (green) and negative control (red).

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human colon tissues using GOLGA2 mouse mAb with DAB staining.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human brain tissues using GOLGA2 mouse mAb with DAB staining.

 

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