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ARG1

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Immunofluorescence analysis of Hela cells using ARG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
  • Flow cytometric analysis of BEL-7402 cells using ARG1 mouse mAb (green) and negative control (red).
  • Flow cytometric analysis of HepG2 cells using ARG1 mouse mAb (green) and negative control (red).

图片

  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Immunofluorescence analysis of Hela cells using ARG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Flow cytometric analysis of BEL-7402 cells using ARG1 mouse mAb (green) and negative control (red).

  • Flow cytometric analysis of HepG2 cells using ARG1 mouse mAb (green) and negative control (red).

Mouse Antibody to ARG1
Catalog#   :  32085
Size/Concentration: 50ul100ul
Price: 12802180
Instruction:
Specification
Entrez GeneID383
Aliasesarginase 1
Clone#3A6A4
MW37 kDa
Host/IsotypeMouse IgG2b
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human ARG1 (AA:1-322) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/200 - 1/1000
FCM1/200 - 1/400
Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

References
1,Medicine (Baltimore). 2020 Aug 7;99(32):e21634.
2,Medicine (Baltimore). 2019 Nov;98(47):e17694.

Diagram
Elisa

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunofluorescence

Immunofluorescence analysis of Hela cells using ARG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Flow cytometric analysis of BEL-7402 cells using ARG1 mouse mAb (green) and negative control (red).

 

Flow cytometric

Flow cytometric analysis of HepG2 cells using ARG1 mouse mAb (green) and negative control (red).

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