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LSD1/AOF2

Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
  • Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining.

图片

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    Western Blot

    Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining.

Mouse Antibody to LSD1/AOF2
Catalog#   :  20055
Size/Concentration： 50ul100μl
Price:￥ 12802180
Instruction:
Specification
Entrez GeneID23028
AliasesKDM1; LSD1; AOF2
Clone#1B2E5
MW93kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Monkey
ImmunogenPurified recombinant fragment of human LSD1 expressed in E. Coli.
FormulationAscitic fluid containing 0.03% sodium azide.
Application
ELISA1/10000
WB1/500 - 1/2000
IHC1/200 - 1/1000
Description
The amine oxidase domain 2 (AOF2) gene encodes a nuclear protein (LSD1, ~95kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex (es) on chromatin.
References
1. Shi YJ, et.al Mol Cell. 2005 Sep 16;19(6):857-64.
2. Metzger E, et.al Nature. 2005 Sep 15;437(7057):436-9.

Diagram
Western Blot

Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining.

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