Mouse TUG
图片
- Western Blot
Western blot analysis using TUG mouse mAb against NIH/3T3 cell lysate.
Mouse Antibody to Mouse TUG
Catalog#
: 20048
| Size/Concentration： | 50ul | 100μl | |
| Price:￥ | 1280 | 2180 |
Instruction: 
Specification
| Entrez GeneID | 79058 |
| Aliases | ASPCR1, ASPL, ASPS, RCC17, TUG, UBXD9, UBXN9 |
| Clone# | 4A11A6G11 |
| Host/Isotype | Mouse IgG1 |
| Storage | 4℃; -20℃ for long term storage. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | Purified recombinant fragment of TUG expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application
| ELISA | 1/10000 |
| WB | 1/500 - 1/2000 |
Description
The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.
References
1. Nature 2003,425:727 - 733.
Sequence
1-258
Diagram
| Western Blot |
Western blot analysis using TUG mouse mAb against NIH/3T3 cell lysate. |