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MSH2

Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.
  • Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.
  • Confocal Immunofluorescence analysis of Hela cells using MSH2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.

图片

  • fff
    Western Blot

    Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.

  • fff
    Immunofluorescence

    Confocal Immunofluorescence analysis of Hela cells using MSH2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.

Mouse Antibody to MSH2
Catalog#   :  20025
Size/Concentration： 50ul100μl
Price:￥ 12802180
Instruction:
Specification
Entrez GeneID4436
AliasesFCC1; COCA1; HNPCC; LCFS2
Clone#1B3A8A8
MW105kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman,Monkey
ImmunogenPurified recombinant fragment of human MSH2 expressed in E. Coli.
FormulationAscitic fluid containing 0.03% sodium azide.
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/200 - 1/1000
IHC1/200 - 1/1000
Description
MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA
replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic
colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal
(HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic
information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI).
These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
References
1. Papadopoulos, N. 1994. Science 263: 1625-1629.
2. Palombo, F. 1994. Nature 367:417-418.
Sequence

2-266


Diagram
Western Blot

Western blot analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.

Immunofluorescence

Confocal Immunofluorescence analysis of Hela cells using MSH2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.

 

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