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BCL-10

Western blot analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.
  • Western blot analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.
  • Confocal Immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.

图片

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    Western Blot

    Western blot analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.

  • fff
    Immunofluorescence

    Confocal Immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

  • fff
    Flow cytometric

    Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.

Mouse Antibody to BCL-10
Catalog#   :  20003
Size/Concentration： 50ul100μl
Price:￥ 12802180
Instruction:
Specification
Entrez GeneID8915
AliasesCLAP; Me10; CIPER; c-E10; CARMEN
Clone#4F8
MW26kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenPurified recombinant fragment of human BCL-10 expressed in E. Coli.
FormulationAscitic fluid containing 0.03% sodium azide.
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/200 - 1/1000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description
Bcl-10 (B-cell CLL/lymphoma 10), also known as CLAP, Me10, CIPER, c-E10, CARMEN. Entrez Protein NP_003912. It is a protein containing a caspase recruitment domain (CARD). It plays an important
role in apoptosis and activating NF-kappaB. The research suggested that it interacted with other
CARD domain containing proteins including CARD9, 10, 11 and 14, which were thought to function as
upstream regulators in NF-kappaB signaling. Bcl-10 is found to form a complex with MALT1 which
encoded by another gene known to be translocated in MALT lymphoma. MALT1 and Bcl-10 are thought to
synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to
the same pathogenetic process that leads to the malignancy.
References
1. Willis, T.G., et al. (1999) Cell. 96, 35-45.
2. Lucas, P.C., et al. (2001) J. Biol.Chem. 276, 19012-19019.
3. Wang, L., et al. (2001) J. Biol.Chem. 276, 21405-21409
Sequence

3-14 (Cys- QSNRELVVDFLS)


Diagram
Western Blot

Western blot analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.

Immunofluorescence

Confocal Immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric

Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).

 

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.

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