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FLAG Tag

Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane: Fusion proteins containing the flag Tag, Primary antibody: P00005(FLAG Tag Antibody) at dilution 1/5000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes
  • Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane: Fusion proteins containing the flag Tag, Primary antibody: P00005(FLAG Tag Antibody) at dilution 1/5000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes

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  • Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane: Fusion proteins containing the flag Tag, Primary antibody: P00005(FLAG Tag Antibody) at dilution 1/5000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes

Rabbit Antibody to FLAG Tag
Catalog#   :  P00005
Size/Concentration: 100ul
Price: 1980
Instruction:
Specification
AliasesDDDDK epitope tag; DYKDDDDK epitope tag
MW50 kDa
Host/IsotypeImmunogen-specific rabbit IgG
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityFusion proteins containing the Flag Tag
ImmunogenSynthetic peptide: DYKDDDDK conjugated to KLH
FormulationpH7.4 PBS, 0.05% NaN3, 40% Glycerol
Application
ELISA1/5000-1/10000
WB1/2000-1/10000
Description
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

Diagram
Western Blot

Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane: Fusion proteins containing the flag Tag, Primary antibody: P00005(FLAG Tag Antibody) at dilution 1/5000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes

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