Specification
Aliases | DDDDK epitope tag; DYKDDDDK epitope tag |
MW | 50 kDa |
Host/Isotype | Immunogen-specific rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Fusion proteins containing the Flag Tag |
Immunogen | Synthetic peptide: DYKDDDDK conjugated to KLH |
Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Application
ELISA | 1/5000-1/10000 |
WB | 1/2000-1/10000 |
Description
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
Diagram
Western Blot |
Gel: 8%SDS-PAGE, Lysate: 0.5 μg, Lane: Fusion proteins containing the flag Tag, Primary antibody: P00005(FLAG Tag Antibody) at dilution 1/5000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 2 minutes |