Specification
Host/Isotype | Immunogen-specific rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | FITC |
Immunogen | KLH conjugated FITC |
Formulation | pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Application
ELISA | 1/5000-1/10000 |
Description
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
Diagram