新品代表
MouseMonoclonalAntibodytoHNF1B
Cat#:32398
Clone#：4H5C2
Host/Isotype：MouseIgG1
EntrezGeneID:6928
MW：61.3kDa
Aliases:T2D;FJHN;HNF2;LFB3;RCAD;TCF2;HPC11;LF-B3;MODY5;TCF-2;VHNF1;ADTKD3;HNF-1B;HNF1beta;HNF-1-beta
SpeciesReactivity：Human;Mouse;Rat
Immunogen：PurifiedrecombinantfragmentofhumanHNF1B(AA:23-132)expressedinE.Coli.
Formulation：PurifiedantibodyinPBSwith0.05%sodiumazide
Storage：4℃;-20℃forlongtermstorage
Application：ELISA1/10000WB1/500-1/2000IHC1/200-1/1000ICC1/50-1/200FCM1/200-1/400
Description：Thisgeneencodesamemberofthehomeodomain-containingsuperfamilyoftranscriptionfactors.TheproteinbindstoDNAaseitherahomodimer,oraheterodimerwiththerelatedproteinhepatocytenuclearfactor1-alpha.Thegenehasbeenshowntofunctioninnephrondevelopment,andregulatesdevelopmentoftheembryonicpancreas.Mutationsinthisgeneresultinrenalcystsanddiabetessyndromeandnoninsulin-dependentdiabetesmellitus,andexpressionofthisgeneisalteredinsometypesofcancer.Multipletranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.[providedbyRefSeq,Sep2009]
抗体应用
Figure1:WesternblotanalysisusingHNF1BmousemAbagainstHela(1)celllysate.
Figure2：ImmunofluorescenceanalysisofHelacellsusingHNF1BmousemAb(green).Blue:DRAQ5fluorescentDNAdye.Red:ActinfilamentshavebeenlabeledwithAlexaFluor-555phalloidin.SecondaryantibodyfromFisher(Cat#:35503)
Figure3：Immunohistochemicalanalysisofparaffin-embeddedmousekidneytissuesusingHNF1BmousemAbwithDABstaining.
Figure4：Immunohistochemicalanalysisofparaffin-embeddedratkidneytissuesusingHNF1BmousemAbwithDABstaining.
Figure5：Immunohistochemicalanalysisofparaffin-embeddedcolontissuesusingHNF1BmousemAbwithDABstaining.
Figure6：Immunohistochemicalanalysisofparaffin-embeddedrectumcancertissuesusingHNF1BmousemAbwithDABstaining.
Figure7:FlowcytometricanalysisofPC-3cellsusingHNF1BmousemAb(green)andnegativecontrol(red).
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