Rabbit Antibody to ACSS2(Phospho-Ser659)
Catalog#
: P44681
| Size/Concentration： | 100μl | |
| Price:￥ | 2180 |
Instruction: 
Specification
| MW | 78kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Hu Ms |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from Human ACSS2 around the phosphorylation site of serine 659. |
Application
| WB | WB 1/500-1/2000 |
Description
Overcoming metabolic stress is a critical step in tumor growth. Acetyl coenzyme A (acetyl-CoA) generated from glucose and acetate uptake is important for his-tone acetylation and gene expression. However, how acetyl-CoA is produced under nutritional stress is un-clear. We demonstrate here that glucose deprivation results in AMP-activated protein kinase (AMPK)-medi-ated acetyl-CoA synthetase 2 (ACSS2) phosphoryla-tion at S659, which exposed the nuclear localization signal of ACSS2 for importin a5 binding and nuclear translocation. In the nucleus, ACSS2 binds to tran-scription factor EB and translocates to lysosomal and autophagy gene promoter regions, where ACSS2 incorporates acetate generated from histone acetylation turnover to locally produce acetyl-CoA for histone H3 acetylation in these regions and pro-mote lysosomal biogenesis, autophagy, cell survival, and brain tumorigenesis. In addition, ACSS2 S659 phosphorylation positively correlates with AMPK ac-tivity in glioma specimens and grades of glioma malig-nancy. These results underscore the significance of nuclear ACSS2-mediated histone acetylation in main-taining cell homeostasis and tumor development.
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