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ATP1A1

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Western blot analysis using ATP1A1 mouse mAb against Hela (1) and A431 (2) cell lysate.
  • Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
  • Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).
  • Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ATP1A1 mouse mAb with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.

图片

  • fff
    Elisa

    Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • fff
    Western Blot

    Western blot analysis using ATP1A1 mouse mAb against Hela (1) and A431 (2) cell lysate.

  • fff
    Immunofluorescence

    Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • fff
    Flow cytometric

    Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ATP1A1 mouse mAb with DAB staining.

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.

Mouse Antibody to ATP1A1
Catalog#   :  32107
Size/Concentration： 50ul100ul
Price:￥ 12802180
Instruction:
Specification
Entrez GeneID476
AliasesCMT2DD; HOMGSMR2
Clone#2D3A1
MW112.8kDa
Host/IsotypeMouse IgG2a
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human ATP1A1 (AA: 153-288) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
WB1/500 - 1/2000
ICC1/200 - 1/1000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

References
1.Autophagy. 2018;14(8):1359-1375.
2.Sci Rep. 2016 Jun 14;6:27738.
Sequence

153-288


Diagram
Elisa

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Western blot analysis using ATP1A1 mouse mAb against Hela (1) and A431 (2) cell lysate.

Immunofluorescence

Immunofluorescence analysis of Hela cells using ATP1A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

 

Flow cytometric

Flow cytometric analysis of Hela cells using ATP1A1 mouse mAb (green) and negative control (red).

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ATP1A1 mouse mAb with DAB staining.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ATP1A1 mouse mAb with DAB staining.

 

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