Specification
Entrez GeneID | 2041 |
Aliases | EPH; EPHT; EPHT1 |
Clone# | 5D2 |
MW | 108kDa |
Host/Isotype | Mouse IgG1 |
Storage | 4℃; -20℃ for long term storage. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of EphA1 expressed in E. Coli. |
Formulation | Purified antibody in PBS containing 0.03% sodium azide. |
Application
ELISA | 1/10000 |
WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
Description
EPH receptor A1 (EphA1), with 976-amino acid protein(about 107 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date and their ligands, the ephrins,can be subdivided into two major subclasses, ephrin-A and ephrin-B. Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior, particularly in the nervous system. They have been implicated in various developmental processes, including axonal guidance, angiogenesis, morphogenesis and carcinogenesis.
References
1. Shannon L. Duffy, Kirsten A. Steiner, Patrick P.L. Tam Gene Expr Patterns. 2006 Feb 6.
2. Elena B. Pasquale. Nat Rev Mol Cell Biol.2005 Jun; 6(6): 462-75.
2. Elena B. Pasquale. Nat Rev Mol Cell Biol.2005 Jun; 6(6): 462-75.
Sequence
38-144
Diagram
Western Blot |
Western blot analysis using EphA1 mouse mAb against A549 (1) and Hela (2) cell lysate. |
Immunohistochemistry |
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma (left) and breast carcinoma (right), showing cytoplasmic localization using EphA1 mouse mAb with DAB staining. |