cTnI
图片
- Immunohistochemistry
Immunohistochemical analysis of paraffin-embedded human normal cardiac muscle tissue, showing cytoplasmic localization using cTnI mouse mAb with DAB staining.
Mouse Antibody to cTnI
Catalog#
: 20050
| Size/Concentration： | 50ul | 100μl | |
| Price:￥ | 1280 | 2180 |
Instruction: 
Specification
| Entrez GeneID | 7137 |
| Aliases | CMH7; RCM1; cTnI; CMD2A; TNNC1 |
| Clone# | 3A10A12 |
| Host/Isotype | Mouse IgG1 |
| Storage | 4℃; -20℃ for long term storage. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of cTnI expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application
| ELISA | 1/10000 |
| IHC | 1/200 - 1/1000 |
Description
cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.
References
1. Cummins B and Cummins P, J Mol Cell Cardiol, 1987, 19(10):999-1010.
2. Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44.
3. Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8.
4. Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8.
5. Adams JE III, Bodor GS, Davila-Roman VG, et al, Circulation, 1993, 88(1):101-6.
2. Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44.
3. Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8.
4. Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8.
5. Adams JE III, Bodor GS, Davila-Roman VG, et al, Circulation, 1993, 88(1):101-6.
Sequence
47-210
Diagram
| Immunohistochemistry |
Immunohistochemical analysis of paraffin-embedded human normal cardiac muscle tissue, showing cytoplasmic localization using cTnI mouse mAb with DAB staining. |
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