CHK1
图片
- Western Blot
Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysate.
Mouse Antibody to CHK1
Catalog#
: 20031
| Size/Concentration： | 50ul | 100μl | |
| Price:￥ | 1280 | 2180 |
Instruction: 
Specification
| Entrez GeneID | 1111 |
| Aliases | CHEK1 |
| Clone# | 2G1D5 |
| MW | 56kDa |
| Host/Isotype | Mouse IgG1 |
| Storage | 4℃; -20℃ for long term storage. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of CHK1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application
| ELISA | 1/10000 |
| WB | 1/500 - 1/2000 |
Description
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
References
1. EMBO J. 1998. 16: 545-554.
2. Science 1998. 282: 1893-1897.
2. Science 1998. 282: 1893-1897.
Sequence
207-421
Diagram
| Western Blot |
Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysate. |
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