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Trim5a

Western blot analysis using Trim5a mouse mAb against human breast carcinoma tissue lysate.
  • Western blot analysis using Trim5a mouse mAb against human breast carcinoma tissue lysate.
  • Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma(A) and stomach adenocarcinoma (B), showing cytoplasmic localization using Trim5a mouse mAb with AEC staining (A) and DAB staining(B).

图片

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    Western Blot

    Western blot analysis using Trim5a mouse mAb against human breast carcinoma tissue lysate.

  • fff
    Immunohistochemistry

    Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma(A) and stomach adenocarcinoma (B), showing cytoplasmic localization using Trim5a mouse mAb with AEC staining (A) and DAB staining(B).

Mouse Antibody to Trim5a
Catalog#   :  20023
Size/Concentration： 50ul100μl
Price:￥ 12802180
Instruction:
Specification
Entrez GeneID85363
AliasesRNF88; TRIM5alpha
Clone#3B11H2
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human trim5 alpha expressed in E. Coli.
FormulationAscitic fluid containing 0.03% sodium azide.
Application
ELISA1/10000
WB1/500 - 1/2000
IHC1/200 - 1/1000
Description
TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5 alpha, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.
References
1. Stremlau, M. Nature 2004.427:848-53.
2. Song, B. J Virol. 2005.79(7):3930-7.

Diagram
Western Blot

Western blot analysis using Trim5a mouse mAb against human breast carcinoma tissue lysate.

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma(A) and stomach adenocarcinoma (B), showing cytoplasmic localization using Trim5a mouse mAb with AEC staining (A) and DAB staining(B).

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