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c-Myc Tag

Gel: 8%SDS-PAGE, Lysate: 0.0016/0.008/0.032 μg, Lane 1-3: 0.0016/0.008/0.032 μg Fusion proteins containing the c-Myc Tag, Primary antibody: P00006(c-Myc Tag Antibody) at dilution 1/8000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds
  • Gel: 8%SDS-PAGE, Lysate: 0.0016/0.008/0.032 μg, Lane 1-3: 0.0016/0.008/0.032 μg Fusion proteins containing the c-Myc Tag, Primary antibody: P00006(c-Myc Tag Antibody) at dilution 1/8000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds

图片

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    Western Blot

    Gel: 8%SDS-PAGE, Lysate: 0.0016/0.008/0.032 μg, Lane 1-3: 0.0016/0.008/0.032 μg Fusion proteins containing the c-Myc Tag, Primary antibody: P00006(c-Myc Tag Antibody) at dilution 1/8000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds

Rabbit Antibody to c-Myc Tag
Catalog#   :  P00006
Size/Concentration： 100ul
Price:￥ 1980
Instruction:
Specification
SwissprotP01106
Aliasesc- Myc epitope Tag
MW55 kDa
Host/IsotypeImmunogen-specific rabbit IgG
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityFusion proteins containing the c-Myc Tag
ImmunogenSynthetic peptide: C-EQKLISEEDL conjugated with KLH.
FormulationpH7.4 PBS, 0.05% NaN3, 40% Glycerol
Application
ELISA1/5000-1/10000
WB1/10000-1/50000
Description
A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Diagram
Western Blot

Gel: 8%SDS-PAGE, Lysate: 0.0016/0.008/0.032 μg, Lane 1-3: 0.0016/0.008/0.032 μg Fusion proteins containing the c-Myc Tag, Primary antibody: P00006(c-Myc Tag Antibody) at dilution 1/8000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds

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