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6×His Tag

Gel: 8%SDS-PAGE, Lysate: 1/0.5/0.5 μg, Lane 1-3: Two fusion protein containing the HIS Tag, Primary antibody: P00002(6×His Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 seconds
  • Gel: 8%SDS-PAGE, Lysate: 1/0.5/0.5 μg, Lane 1-3: Two fusion protein containing the HIS Tag, Primary antibody: P00002(6×His Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 seconds

图片

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    Western Blot

    Gel: 8%SDS-PAGE, Lysate: 1/0.5/0.5 μg, Lane 1-3: Two fusion protein containing the HIS Tag, Primary antibody: P00002(6×His Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 seconds

Rabbit Antibody to 6×His Tag
Catalog#   :  P00002
Size/Concentration： 100ul
Price:￥ 1980
Instruction:
Specification
Aliases6 His epitope tag
MW50/44 kDa
Host/IsotypeImmunogen-specific rabbit IgG
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityFusion proteins containing the HIS Tag
ImmunogenSynthetic peptide: 18×His conjugated to KLH
FormulationpH7.4 PBS, 0.05% NaN3, 40% Glycerol
Application
ELISA1/5000-1/10000
WB1/2000-1/10000
Description
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Diagram
Western Blot

Gel: 8%SDS-PAGE, Lysate: 1/0.5/0.5 μg, Lane 1-3: Two fusion protein containing the HIS Tag, Primary antibody: P00002(6×His Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 seconds

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