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C5AR2

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
  • Immunofluorescence analysis of Hela cells using C5AR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
  • Flow cytometric analysis of Molt4 cells using C5AR2 mouse mAb (green) and negative control (red).
  • Flow cytometric analysis of RAW24.7 cells using C5AR2 mouse mAb (green) and negative control (red).
  • Flow cytometric analysis of HL-60 cells using C5AR2 mouse mAb (green) and negative control (red).
  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using C5AR2 mouse mAb with DAB staining.

图片

  • Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

  • Immunofluorescence analysis of Hela cells using C5AR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

  • Flow cytometric analysis of Molt4 cells using C5AR2 mouse mAb (green) and negative control (red).

  • Flow cytometric analysis of RAW24.7 cells using C5AR2 mouse mAb (green) and negative control (red).

  • Flow cytometric analysis of HL-60 cells using C5AR2 mouse mAb (green) and negative control (red).

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using C5AR2 mouse mAb with DAB staining.

Mouse Antibody to C5AR2
Catalog#   :  32082
Size/Concentration: 50ul100ul
Price: 12802180
Instruction:
Specification
Entrez GeneID27202
AliasesC5L2; GPF77; GPR77
Clone#6C1G10
MW36kDa
Host/IsotypeMouse IgG1
Storage4℃; -20℃ for long term storage. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human C5AR2 (AA: extra mix) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide
Application
ELISA1/10000
ICC1/200 - 1/1000
FCM1/200 - 1/400
IHC1/200 - 1/1000
Description

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants.

References
1,Oncotarget. 2017 Jan 31;8(5):8590-8596.
2,J Dent Res. 2017 Jan;96(1):92-99.
Sequence

extra mix


Diagram
Elisa

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Immunofluorescence

Immunofluorescence analysis of Hela cells using C5AR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Flow cytometric analysis of Molt4 cells using C5AR2 mouse mAb (green) and negative control (red).

 

Flow cytometric

Flow cytometric analysis of RAW24.7 cells using C5AR2 mouse mAb (green) and negative control (red).

Flow cytometric

Flow cytometric analysis of HL-60 cells using C5AR2 mouse mAb (green) and negative control (red).

Immunohistochemistry

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using C5AR2 mouse mAb with DAB staining.

 

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